When the hydrogen peroxide is dripped on to the guaiac paper, it oxidizes the alpha-guaiaconic acid to a blue colored quinone.
2.
The test involves placing a faecal sample on "'guaiac "'paper ( containing a phenolic compound, alpha-guaiaconic acid, extracted from the wood resin of Guaiacum trees ) and applying hydrogen peroxide which, in the presence of blood, yields a blue reaction product within seconds.
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